Liver damage in acute heliotrine poisoning. 1. The intracellular distribution of pyridine nucleotides

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Liver Damage in Acute Heliotrine Poisoning 2. THE EFFECT OF NICOTINAMIDE ON PYRIDINE NUCLEOTIDE

Earlier studies on the effects of heliotrine poisoning demonstrated an apparent deficiency of DPN in homogenate and mitochondrial preparations from the livers of heliotrine-poisoned (1620hr.) rats (Christie, 1958b). More recent work has shown that the concentrations of pyridine nucleotides were, in fact, decreased in homogenates and in the mitochondrial and soluble fractions of liver from rats ...

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Streptozotocin: depression of mouse liver pyridine nucleotides.

phenomenon readily prevented by nicotinamide pretreatment (21). This communication deals with the changes in liver NAD and reduced nicotinamide adenine dinucleotide (NADH) con centrations over a 48-hour period following the single intra venous administration of Streptozotocin. These effects are cor related with the measurement of drug concentration in serum and the acid-soluble fraction of live...

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Distribution of Enzymes Cleaving Pyridine Nucleotides in Animal Tissues

1. The distribution of DPN and DPNH pyrophosphatases and DPNase in centrifugally prepared fractions of organs of several species of animals is reported. 2. A DPNH pyrophosphatase was found in the soluble fraction of pigeon and of rabbit liver. This enzyme did not split DPN but accounted for over 50 per cent of the DPNH pyrophosphatase activity of the whole homogenates. 3. All the organs tested,...

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The stability of pyridine nucleotides.

Diphosphopyridine nucleotide and triphosphopyridine nucleotide when used in appropriate enzyme systems become exceedingly useful reagents for the measurement of almost any biochemical substance. A companion paper describes procedures for measuring each of the pyridine nucleotides, whether in the oxidized or reduced form, at concentrations as low as 1O-g M and in amounts as small as lo-l5 moles ...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1962

ISSN: 0006-2936

DOI: 10.1042/bj0840025